使用機械分散機(Dispermat)混合疏水蛋白,SDS和纖維。分散機速度可調,但通常在實驗中使用約3800rpm。研究泡沫體積隨時間的變化。通過此設置,發(fā)泡在1分鐘內(nèi)達到最大水平。通過攝影監(jiān)測泡沫體積的變化,其中每10秒拍攝照片。從照片圖像中,確定達到50%氣體體積(t50)和66%氣體體積(t66)所需的混合時間。此外,測定達到的最大體積(Omax)。記錄10分鐘測量泡沫的塌陷率,而每1分鐘拍攝照片。根據(jù)這些數(shù)據(jù),可以估算泡沫的V /。 V /值描述當液體體積達到其初始高度的一半(排水量)時停止混合后經(jīng)過的時間。
Mechanical mixer (Dispermat) was used to mix hydrophobin, SDS and fibers. Mixer speed was adjustable, but generally about 3800 rpm used in the experiments. Foam volume was studied as a function of time. Foaming reached the maximum level within 1 min with this set-up. The change of foam volume was monitored by photography,where pictures were taken every 10 s. From the photographic images, the mixing times required for reaching 50% gas volume (t50) and 66% gas volume (t66) were determined. In addition, the maximal volume achieved (Omax) was determined.Collapse rate of foam was measured with 10 min recording, whereas pictures were taken every 1 min. From these data V/ for the foam can be estimated. The V/ value describes the time elapsed after cessation of mixing when liquid volume reaches half of its initial height (drainage volume).